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Housing laboratory animals under lighting conditions that more closely mimic the natural environment may improve their wellbeing. This study examined the effects of dim light or a long-night photocycle on resting heart rate HR of rats and their HR responses to acute procedures. Male and female Sprague—Dawley SD and spontaneously hypertensive SHR rats, instrumented with radiotelemetry transmitters and housed individually under a h light:dark photocycle with 10 lx illumination dim light or under an h light:dark photocycle with lx illumination long nights , were compared with control rats individually housed under a h light:dark photocycle with lx illumination. When rats were subjected acutely to husbandry, experimental, or stressful procedures, dim light or long nights or both reduced HR responses to some procedures, did not alter responses to others, and increased responses to yet other procedures.

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